10% PVP in FertiCult Flushing medium
Description
Intra-cytoplasmatic sperm injection (ICSI) requires the capture of individual sperm cells in a glass pipette for injection into the oocyte. This procedure is facilitated by first immobilizing the sperm.
The most widely used method of immobilizing the sperm cells is by placing them in a viscous medium prior to nicking the tail to immobilize sperm completely.
Product specifications
10% PVP in FertiCult Flushing medium contains 4 g/Liter Human Serum Albumin (HSA) to optimize medium performances. HSA is universally added to most ART media because it is widely considered to be of benefit.
The role of albumin in ART media is extensive, including:
– Stabilization of the cell membrane of the embryo in the medium (Malda, et al. 2008).
– Inhibition of lipid peroxidation that can be damaging to sperm (Alvarez and Storey 1995).
