Hyaluronidase in FertiCult Flushing medium
Description
Hyaluronidase in FertiCult Flushing medium contains 80 IU/ml hyaluronidase from bovine origin, in FertiCult Flushing medium (HEPES-buffered medium which contains 4.00g/liter human serum albumin). The product is ready for use. Information about the composition of the product can be found in the material safety data sheet.
Hyaluronidase in FertiCult Flushing medium is used in the oocyte denudation process. Hyaluronidase digests the hyaluronic acid between the cumulus cells, which makes it easier to remove the cumulus mechanically.
Product specifications
Hyaluronidase in FertiCult Flushing medium contains 4 g/Liter Human Serum Albumin (HSA) to optimize medium performances. HSA is universally added to most ART media because it is widely considered to be of benefit.
The role of albumin in ART media is extensive, including:
– Stabilization of the cell membrane of the embryo in the medium (Malda, et al. 2008).
– Inhibition of lipid peroxidation that can be damaging to sperm (Alvarez and Storey 1995).
