SpermFreeze
Description
SpermFreeze is a single step freezing medium for use with human sperm cells. The medium is clear (does not contain milk, egg yolk, …) and can be used direct from the bottle.
SpermFreeze requires 0.7mL of medium for each millilitre of semen.
We also offer a more concentrated SpermFreeze which allows 3mL of semen to be cryopreserved with 1mL of SpermFreeze SSP.
Product specifications
Cryoprotectants act by decreasing the freezing point of a substance, reducing the amount of salts and solutes present in the liquid phase of the sample and by decreasing ice formation within the spermatozoa. Importantly, the cryoprotectant should be non-toxic to the cells during the addition of the cryoprotectant and during freezing and thawing.
Glycerol is a permeating cryoprotectant which is most widely used for human sperm cryopreservation and meets all criteria indicated above.
